Novel Primers-Probe Sets for Detection and Identification of Mycobacteria by PCR-Microarray Assay

摘要

A PCR-Microarray assay was developed in which PCR primers and hybridization probes were designed on the 16S rRNA genes of 16 clinically relevant mycobacteria and on IS6110 of Mycobacterium tuberculosis. The assay, based on a multiplex PCR followed by hybridization with oligonucleotide probes, was tested against 16 Mycobacterium species and 70 clinical samples.